Materials and Biophysical Chemistry Laboratory

Yale Chemistry 335L: Materials and Biophysical Chemistry Laboratory offers six modules covering the synthesis of graphene oxide nanosheets, growth of quantum dots on graphene, solid-state synthesis of terbium-doped lanthanum oxysulfide, and the microscopy modules listed below. The course was co-developed and co-taught with Prof. Hailiang Wang. Lab manuals developed by ZG are available for free download below; these manuals provide scientific background, a parts list, and step-by-step instructions for constructing each instrument.


Module 1: Microscopy and Image Resolution.

Download Lab Manual for Microscopy and Image Resolution here. This module will review geometric optics, Fourier transform relationships between image and aperture planes, and resolution as they pertain to microscopy. Students will build a microscope, characterize its resolution and image aberrations, write software to process images, and learn about different image collection modalities.

Module 2: Single Molecule Fluorescence.

Download Lab Manual for Single Molecule Fluorescence here. This module introduces students to single molecule fluorescence using semiconductor nanoparticles (quantum dots). Basic solid state theory and quantum models of semiconductor structure will be discussed. Students will characterize the electronic structure, photoluminescence, blinking behavior of quantum dots, and relaxation mechanisms of excited electronic states.  Applications of quantum dots as specific and size-tunable fluorescence markers will be discussed.

Module 3: Optical Trapping.

Download Lab Manual for Optical Trapping here. In this module, students will learn the principles behind the optical trapping methods that underlie single molecule force manipulation techniques. They will construct an optical trap and calibrate its force generation. The effects of laser wavelength/particle diameter, and medium viscosity on the trapping stiffness will be characterized. Students will measure the compliance of bacterial flagella motors.


References:

Handbook of optics [electronic resource] / sponsored by the Optical Society of America

Handbook of Biological Confocal Microscopy [electronic resource] / edited by James B. Pawley

Principles of Fluorescence Spectroscopy [electronic resource] / edited by Joseph R. Lakowicz

Single Molecule Analysis [electronic resource] / edited by Erwin J. G. Peterman, Gijs J. L. Wuite